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Alpha-1 Antitrypsin (A1AT) Phenotype Testing

The reference method for the Alpha-1 Antitrypsin (A1AT) phenotype testing.

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How do we diagnose A1AT Deficiency?

The most common method to detect A1AT Deficiency is protein phenotyping using isoelectric focusing.

The HYDRAGEL 18 A1AT ISOFOCUSING kit used on the HYDRASYS 2 Scan Focusing instrument provides qualitative detection of the different phenotypes of the A1AT in human serum.

The semi-automated HYDRASYS 2 instrument enables migration, immunofixation and direct detection on agarose gels, to obtain A1AT profiles for qualitative analysis.

Phenotyping results in conjunction with clinical findings and other laboratory assays aid in the diagnosis of Alpha-1 Antitrypsin Deficiency.

Sebia A1AT Gel, provided by the HYDRASYS 2 SCAN FOCUSING instrument

Pathologies associées

Technologies utilisées

Key benefits

Provides a clear and comprehensive characterization of A1AT phenotypes associated with Alpha-1 Antitrypsin Deficiency, a common genetic disorder that is frequently underrecognized and diagnosed late.

  • Accurate Diagnosis

    Designed for the qualitative detection and identification of A1AT phenotypes in serum samples. Achieved 100% of concordance with an established agarose gel isoelectric focusing method, and in agreement across all phenotypes evaluated.

  • Efficient Method

    Clear bands visualization for a reliable interpretation.

  • Fast Result

    Simple step-by-step procedure without membrane transfer for 2.5 hours of turnaround time.

  • Testing is part of algorithms

    HYDRAGEL 18 A1AT ISOFOCUSING is included in both screening and diagnostic algorithms to provide clinicians with phenotype of A1AT proteins secreted in blood circulation.

  • Detection limit of an Alpha-1 Antitrypsin’s isoform:

    5 mg/dL

Main characteristics

Test principle

The HYDRAGEL 18 A1AT ISOFOCUSING assay is based on the principle of agarose gel isoelectrofocusing (IEF) followed by immunofixation.

  • After the protein separation according to their isoelectric point, the gel is directly incubated with a peroxidase labelled anti-A1AT antiserum with no need for a membrane blotting step.
  • Then, the gel is processed in order to remove unbound proteins before the enzymatic reaction.
  • Finally, the gel is visually evaluated.

Sample type

Human serum samples can be used with HYDRAGEL 18 A1AT ISOFOCUSING.

Performances

  • 2 stages-protocol: Isoelectrofocalisation and Immunofixation for an optimized result.
  • A1AT controls (MM, MZ and MS) are included in each series of analysis to quickly identify M, S and Z phenotypes.

Product details

10 gels of 18 tests.

Reagents

  • HYDRAGEL 18 A1AT ISOFOCUSING – P/N: 4357

Accessories

  • ANTI-A1AT - PER – P/N: 4746

Knowledge & Science

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A1AT test can be performed on the following instrument

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