Serum and Urine Immunotyping

Fully automated monoclonal proteins identification by Capillarys electrophoresis.

Published date: 3/8/2021 | Modified date: 6/9/2026

About Serum and Urine Immunotyping

Immunotyping is used to detect and identify monoclonal proteins in serum and urine. Serum and urine proteins are mixed with specific antisera against Gamma- (IgG), Alpha- (IgA), Mu- (IgM) heavy chains Kappa (κ) and Lambda (λ) free and bound light chains.

Immunoglobulins specifically react with their corresponding antiserum and each antiserum pattern (IgG, IgA, IgM, κ and λ) is automatically overlaid with the ELP (Protein Electrophoresis) reference curve. Disappearance of the abnormality in the antiserum-treated pattern indicates the presence of a monoclonal protein.

Key benefits

Serum and Urine Immunotyping powered by Capillary Electrophoresis.

Fully automated testing
Qualitative analysis with fast separation and good resolution of serum and urine samples.
Direct visualisation of serum protein profile
for quick identification of suspect monoclonal proteins (IgG, IgA, IgM, Kappa (κ) and Lambda (λ)) involved in gammopathy.
High quality of antisera ready to use
for an optimum reaction performance and correct monoclonal component identification.
Scalable solutions
to best fit the need for all sized laboratories, from a standalone instrument to workcell configuration.

Main characteristics

Test principle

Based on the principle of capillary electrophoresis in free solution. Serum or Urine proteins are mixed with specific antisera.
Immunoglobulins specifically react with their corresponding antiserum. At the end of the analysis, each antiserum pattern (IgG, IgA, IgM, κ and λ) is automatically overlaid with the ELP (Protein Electrophoresis) reference curve.
Disappearance of the abnormality in the antiserum-treated pattern indicates the presence of a monoclonal protein.

Sample type

Serum & Urine

Performances

Full traceability and positive sample ID from primary tube samples
Full traceability and reagents management through RFID tags for both reagents and antisera
On board antisera vials kept at cool temperature
Full automation
Color-coded backlit reagent containers

Sensitivity

Monoclonal protein can be detected at a concentration as low as 12.5 mg/dL in serum. According to the mobility of the monoclonal component and polyclonal background, the sensitivity may vary.
Protein abnormality limit of detection is approximately 9 mg/L with CAPI 3 URINE.

Throughput

Throughput (tests/hour)*

IMMUNOTYPING

CAPILLARYS 3 OCTA (serum or urine) 9
CAPILLARYS 3 TERA (serum or urine) 12
CAPILLARYS 3 TERA MC3** (serum or urine) 34
MINICAP FLEX-PIERCING (serum) 2

*Firmware-dependent; contact your local Sebia representative for current information.
**Maximum Capacity or MC designation refers to Tube Loader CAPILLARYS 3 - P/N: 1249 and Rack Conveyor CAPILLARYS 3 - P/N: 1250 associated with CAPILLARYS 3 TERA - P/N: 1246.

Product details

CAPI 3 IMMUNOTYPING – P/N: 2600
MINICAP IMMUNOTYPING – P/N: 2300

Knowledge & Science

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