Easy to Use
Once the samples are punched and the plates are loaded, the instrument fully automates all steps, making it easy to operate and requiring minimal maintenance.
Newborn Hemoglobin disorders screening
The perfect match for screening Hemoglobin Disorders in newborns.
How do we diagnose Newborn Hemoglobin Disorder?
Each day, more than 100,000 newborns worldwide are screened for metabolic, endocrine, and genetic disorders. While hemoglobin screening has a long-standing history in some countries, such as the USA, France and the UK, it has only recently been incorporated into newborn screening programs in others, including Portugal, Germany, and Australia.
The rising prevalence of hemoglobin disorders in regions where they were previously uncommon has increased awareness of the need for newborn screening.
Early detection has been shown to improve the lives of babies by enabling timely treatment and appropriate follow-up care.
The CAPI 3 NEONAT Hb kit is intended for the detection of normal hemoglobins (F and A) and abnormal hemoglobins (S, C, E, D and Bart’s) in blood from human newborn collected on filter paper. This qualitative analysis is performed by Capillary Electrophoresis with the CAPILLARYS 3 DBS automated instrument. The test result must be interpreted in conjunction with other biological and clinical findings.
Pathologies associées
Technologies utilisées
Key benefits
The CAPILLARYS 3 DBS instrument, the latest, user-friendly, high-throughput solution for detecting hemoglobin variants and screening thalassemias in newborns.
High Autonomy & Throughput
Between one and eight full 96-well microplates can be processed in a single run, with a throughput of 74 tests per hour. Load-and-go workflow improves efficiency and reduces hands-on time.
Full traceability
From sample punching with an automatic puncher to the export of results from the CE instrument software, patient information is automatically transferred via the sample's barcode, eliminating the risk of manual errors during ID entry.
High Resolution
Thanks to the high-resolution hemoglobin separation provided by the Sebia CAPILLARYS 3 DBS device, the instrument produces a clear profile that facilitates the identification of abnormal hemoglobin variants (Hb S, C, D, E, etc.) as well as thalassemia-related abnormalities such as Hb H and Hb Bart’s.
Automatic Result Interpretation
The software automatically classifies samples using a color-coding system to distinguish between normal or abnormal results, allowing laboratories to quickly identify pathological findings while quickly releasing non-pathological samples.
Main characteristics
Test principle
- The NEONAT Hb assay is based on the principle of Capillary Electrophoresis in free solution. Hemoglobin fractions are separated in silica capillaries, by their electrophoretic mobility and electroosmotic flow at a high voltage in an alkaline buffer.
- The diluent is formulated to counteract the hemoglobin oxidation in the blood spot, that occurs during drying, room-temperature transportation, and extended storage prior to analysis.
- Hemoglobin fractions are directly detected at the specific absorbance of 415 nm. The resulting pattern is divided into 13 zones to facilitate interpretation of normal hemoglobins (F and A) and abnormal hemoglobin (S, C, D, E and Bart’s).
Sample type
Dried neonatal blood spots are punched (standard size of 3.2 mm), eluted in distilled water, and directly analysed using the CAPILLARYS 3 DBS instrument.
Performances
| CAPI 3 NEONAT Hb | Throughput (tests/hour)* |
|
74 |
*Firmware-dependent; contact your local Sebia representative for current information.
Product details
Dedicated reagent kit, Hemolysing solution and Hb AF control.
Reagents
- CAPI 3 NEONAT Hb – P/N: 2506
- CAPI 3 NEONAT Hemolysing solution – P/N: 2563
Controls
- CAPI 3 NEONAT Hb AF Control – P/N: 4757
- Hb AFSC Control – P/N: 4792 (optional pathological control)
Consumables
- CAPILLARYS 3 DBS Microplates – P/N: 2578 (10 units – 96 well barcoded microplates)
Knowledge & Science
Newborn Hemoglobin Disorder Screening test can be performed on the following instrument
A high-throughput Capillary Electrophoresis solution for newborn screening of hemoglobin disorders.
Test related to Newborn Hemoglobin Disorder Screening
Cette section contient des informations destinées à une large diffusion et peut donc comporter des détails sur des produits ou des informations qui ne sont pas disponibles ou valables dans votre pays. Veuillez contacter votre représentant Sebia local. Informations destinées aux professionnels de santé. Lisez attentivement les instructions figurant dans les notices des réactifs et les manuels d'utilisation des instruments.